Yeast Protocols (e-book) (used book) | bookbook.eu

Description

1. Basic Investigations in Saccharomyces cerevisiae

Brendan Curran and Virginia Bugeja

2. Isolation of Yeast Nucleic Acids

Michael Biss, Michelle Hanna and Wei Xiao

3. Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry

Ting Wei and Heping Dai

4. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

R. Daniel Gietz

5. Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe

Johannes H. Hegemann, Sven Boris Heick, Jennifer Pöhlmann, Markus Langen and Ursula Fleig

6. See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry

Changhui Deng and Andrew N. Krutchinsky

7. Yeast Two Hybrid Liquid Screening

Elisa Donnard, Erica M. Queiroz, J. Miguel Ortega, and R. Daniel Gietz

8. Targeted Mutagenesis of a Specific Gene in Yeast

Likui Zhang, Alina Radziwon, and Linda J. Reha-Krantz

9. Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae

Kai Stefan Dimmer

10. Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy

Wei-Pang Huang, Takahiro Shintani, and Zhiping Xie

11. Assays for Autophagy II: Mitochondrial Autophagy

Tomotake Kanki and Koji Okamoto

12. Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction

Danielle Hagstrom and Changle Ma

13. Yeast Survival and Growth Assays

Xin Xu, Amanda Lambrecht, and Wei Xiao

14. Spontaneous Mutagenesis Assay

Susan Blackwell, Michelle Hanna, and Wei Xiao

15. Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene

Bernard A. Kunz

16. piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe

Jun Li and Li-Lin Du

17. Replicative and Chronological Life Span Assays

Spike D. L. Postnikoff and Troy A. A. Harkness

18. Detection of Protein Arginine Methylation in Saccharomyces cerevisiae

Christopher Aa. Jackson and Michael C. Yu

19. Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae

Lindsay G. Ball and Wei Xiao

20. Ligand Engineering Using Yeast Surface Display

Benjamin J. Hackel

21. Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay

Kris Barreto and C. Ronald Geyer