The Mitotic Spindle (e-book) (used book) | bookbook.eu

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Part I Methods Focused on Cytology

1: Using Fluorescence Microscopy to Study MitosisSai K. Balchand, Barbara J. Mann, and Patricia Wadsworth

2: Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation
Bin He and Daniela Cimini

Part II Methods Focused on Microtubules and the Mitotic Spindle

3: Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts
Aaron C. Groen and Timothy J. Mitchison

4: Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro
Marija Zanic

5: Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles
Nicolas Lecland and Jens Lüders

6: Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy
Matthew King and Sabine Petry

7: Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size
Matthew C. Good

Part III Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface

8: In Vitro Kinetochore Assembly
Matthew D. D. Miell and Aaron F. Straight

9: Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes
Tatsuya Nishino and Tatsuo Fukagawa

10: Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells
Keith F. DeLuca, Jacob A. Herman, and Jennifer G. DeLuca

11: Studying Kinetochores In Vivo Using FLIM-FRET
Tae Yeon Yoo and Daniel J. Needleman

Section IV Methods Focused on the Spindle Pole

12: Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies
Kimberly K. Fong, Beth Graczyk, and Trisha N. Davis

13: A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion
Rajesh Kumar Soni and Meng-Fu Bryan Tsou

14: Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation
Vladimir Joukov, Johannes C. Walter, and Arcangela De Nicolo

Part V Methods Focused on the Cellular Functions of Microtubule Motor Proteins

15: Analyzing Spindle Positioning Dynamics in Cultured Cells
Tomomi Kiyomitsu

16: Quantification of Mitotic Chromosome Alignment
Cindy Fonseca and Jason Stumpff

17: Imaging Mitosis in the Moss Physcomitrella Patens
Moé Yamada, Tomohiro Miki, and Gohta Goshima

18: Small Molecule Approach to Study the Function of Mitotic Kinesins
Naowras Al-Obaidi, Johanna Kastl, and Thomas U. Mayer

Part VI Novel Approaches to Study Spindle Function and Regulation

19: Identification and Characterization of Mitotic Spindle-Localized Transcripts
Amy B. Emerman, Ashwini Jambhekar, and Michael D. Blower

20: Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization
Edward R. Ballister and Michael A. Lampson

21: Studying Kinetochore Kinases
Adrian T. Saurin and Geert J.P.L. Kops

22: Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics
Mathew J.K. Jones and Prasad V. Jallepalli

Part VII The Mitotic Spindle and Cancer

23: Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer
Mijung Kwon

24: Gene