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T-Cell Trafficking: Methods and Protocols Edited by Federica M. Marelli-Berg and Sussan Nourshargh Table of Contents Preface Contributors Introductory review: 1. How T cells find their way around Alf Hamann Migration of T cells in vitro 2. Live imaging of leukocyte-endothelium interactions Olga Barreiro, Francisco Sánchez-Madrid, and MarÃa Yánez-Mó 3. Leukocyte adhesion under haemodynamic flow conditions Charlotte Lawson, Marlene Rose and Sabine Wolf 4. Influence of stromal cells on lymphocyte adhesion and migration on endothelial cells Helen M. McGettrick PhD, Chris D. Buckley PhD, G. Ed Rainger PhD and Gerard B. Nash PhD 5. Discriminating between the paracellular and transcellular routes of diapedesis Eva Cernuda-Morollón, Severine Gharbi, Jaime Millán 6. Monitoring RhoGTPase activity in lymphocytes Marouan Zarrouk, David Killock, Aleksandar Ivetic 7. Visualisation of Signalling in Immune Cells Leo M. Carlin, Konstantina Makrogianneli, Melanie Keppler, Gilbert O. Fruhwirth and Tony Ng 8. Methods for quantisation of lymphocyte chemotaxis and fugetaxis Fabrizio Vianello, Elda Righi and Mark C. Poznansky 9. Analysis of CXCR3 and Atypical Variant Expression and Signalling in Human T Lymphocytes Anna Korniejewska, Malcolm Watson, Stephen Ward 10. Transfection of indoleamine 2,3 dioxygenase in primary endothelial cells Petros XE Mouratidis, Andrew J T George Migration of T cells in vivo 11. Visualisation of lymphoid organ development Henrique Veiga-Fernandes, Katie Foster, Amisha Patel, Mark Coles, and Dimitris Kioussis 12. Single Cell Analysis of Cytotoxic T Cell Function by Intravital Multiphoton Microscopy Thorsten R. Mempel 13. Imaging interactions between the immune and cardiovascular systems in vivo by multiphoton microscopy Owain R. Millington, James M. Brewer, Paul Garside, and Pasquale Maffia 14. Applying an adaptive watershed to the tissue cell quantification during T-cell migration and embryonic development D. Zhu, S. Jarmin, A. Ribeiro, F. Prin, S.Q. Xie, K. Sullivan, J. Briscoe, A.P. Gould, F.M. Marelli-Berg & Y. Gu Monitoring T cell migration in human diseases 15. Identifying homing interactions in T cell traffic in human disease Patricia F. Lalor, Stuart M. Curbishley and David H. Adams 16. Tracking antigen experienced effector T cells in vitro and in vivo Claire L. Gorman, Claudia Monaco, Enrico Ammiratti, Anna-Chiara Vermi, Federica Marelli-Berg and Andrew P. Cope 17. Pre-clinical testing of strategies for therapeutic targeting of human T cell trafficking in vivo Caroline Coisne and Britta Engelhardt
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T-Cell Trafficking: Methods and Protocols Edited by Federica M. Marelli-Berg and Sussan Nourshargh Table of Contents Preface Contributors Introductory review: 1. How T cells find their way around Alf Hamann Migration of T cells in vitro 2. Live imaging of leukocyte-endothelium interactions Olga Barreiro, Francisco Sánchez-Madrid, and MarÃa Yánez-Mó 3. Leukocyte adhesion under haemodynamic flow conditions Charlotte Lawson, Marlene Rose and Sabine Wolf 4. Influence of stromal cells on lymphocyte adhesion and migration on endothelial cells Helen M. McGettrick PhD, Chris D. Buckley PhD, G. Ed Rainger PhD and Gerard B. Nash PhD 5. Discriminating between the paracellular and transcellular routes of diapedesis Eva Cernuda-Morollón, Severine Gharbi, Jaime Millán 6. Monitoring RhoGTPase activity in lymphocytes Marouan Zarrouk, David Killock, Aleksandar Ivetic 7. Visualisation of Signalling in Immune Cells Leo M. Carlin, Konstantina Makrogianneli, Melanie Keppler, Gilbert O. Fruhwirth and Tony Ng 8. Methods for quantisation of lymphocyte chemotaxis and fugetaxis Fabrizio Vianello, Elda Righi and Mark C. Poznansky 9. Analysis of CXCR3 and Atypical Variant Expression and Signalling in Human T Lymphocytes Anna Korniejewska, Malcolm Watson, Stephen Ward 10. Transfection of indoleamine 2,3 dioxygenase in primary endothelial cells Petros XE Mouratidis, Andrew J T George Migration of T cells in vivo 11. Visualisation of lymphoid organ development Henrique Veiga-Fernandes, Katie Foster, Amisha Patel, Mark Coles, and Dimitris Kioussis 12. Single Cell Analysis of Cytotoxic T Cell Function by Intravital Multiphoton Microscopy Thorsten R. Mempel 13. Imaging interactions between the immune and cardiovascular systems in vivo by multiphoton microscopy Owain R. Millington, James M. Brewer, Paul Garside, and Pasquale Maffia 14. Applying an adaptive watershed to the tissue cell quantification during T-cell migration and embryonic development D. Zhu, S. Jarmin, A. Ribeiro, F. Prin, S.Q. Xie, K. Sullivan, J. Briscoe, A.P. Gould, F.M. Marelli-Berg & Y. Gu Monitoring T cell migration in human diseases 15. Identifying homing interactions in T cell traffic in human disease Patricia F. Lalor, Stuart M. Curbishley and David H. Adams 16. Tracking antigen experienced effector T cells in vitro and in vivo Claire L. Gorman, Claudia Monaco, Enrico Ammiratti, Anna-Chiara Vermi, Federica Marelli-Berg and Andrew P. Cope 17. Pre-clinical testing of strategies for therapeutic targeting of human T cell trafficking in vivo Caroline Coisne and Britta Engelhardt
Reviews