Spatial Genome Organization (e-book) (used book) | bookbook.eu

Description

Part I: Sailing the '3Cs': Chromosome Conformation Capture and Its Variants

1. Quantitative Chromosome Conformation Capture (3C-qPCR)

Cosette Rebouissou, Séphora Sallis, and Thierry Forné

2. Detection of Allele-Specific 3D Chromatin Interactions Using High-Resolution In-Nucleus 4C-Seq

Mélanie Miranda, Daan Noordermeer, and Benoit Moindrot

3. Tough Tissue Hi-C

Stefan Grob

4. Mapping Mammalian 3D Genomes by Micro-C

Elena Slobodyanyuk, Claudia Cattoglio, and Tsung-Han S. Hsieh

Part II: Targeted Hi-C Approaches

5. Targeted Chromosome Conformation Capture (HiCap)

Artemy Zhigulev and Pelin Sahlén

6. Assessment of Higher-Order, Multi-Way Interactions with Tri-C

A. Marieke Oudelaar, Damien J. Downes, and Jim R. Hughes

7. Assessing Specific Networks of Chromatin Interactions with HiChIP

Dafne Campigli Di Giammartino, Alexander Polyzos, and Effie Apostolou

Part III: Sequencing-Based Approaches to Assess Nuclear Environment

8. Measuring Cytological Proximity of Chromosomal Loci to Defined Nuclear Compartments with TSA-Seq

Liguo Zhang, Yu Chen, and Andrew S. Belmont

9. The High-Salt Recovered Sequence-Sequencing (HRS-seq) Method: Exploring Genome Association with Nuclear Bodies

Cosette Rebouissou, Marie-Odile Baudement, and Thierry Forné

Part IV: Single-Cell Approaches

10. High-Throughput Preparation of Improved Single-Cell Hi-C Libraries Using an Automated Liquid Handling System

Wing Leung and Takashi Nagano

11. Simultaneous Quantification of Spatial Genome Positioning and Transcriptomics in Single Cells with scDam&T-Seq

Silke J.A. Lochs and Jop Kind

Part V: Visualizing Spatial Genome Organization

12. High-Throughput DNA FISH (hiFISH)

Elizabeth Finn, Tom Misteli, and Gianluca Pegoraro

13. Versatile CRISPR-Based Method for Site-Specific Insertion of Repeat Arrays to Visualize Chromatin Loci in Living Cells

Thomas Sabate, Christophe Zimmer, and Edouard Bertrand

Part VI: Functionally Dissecting Chromatin Architecture

14. CLOuD9: CRISPR-Cas9-Mediated Technique for Reversible Manipulation of Chromatin Architecture

Wei Qiang Seow, Poonam Agarwal, and Kevin C. Wang

15. Acute Protein Depletion Strategies to Functionally Dissect the 3D Genome

Michela Maresca, Ning Qing Liu, and Elzo de Wit