837,69 €

Single-Domain Antibodies

837,69 €

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Publisher: Humana
ISBN-10: 1071620746
ISBN-13: 9781071620748
Format: 18.3 x 26.2 x 3.3 cm, hardcover
Language: English

Single-Domain Antibodies (e-book) (used book) | bookbook.eu

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Description

Part I: Introduction to Single-Domain Antibodies

1. Nanobodies: From Serendipitous Discovery of Heavy Chain-Only Antibodies in Camelids to a Wide Range of Useful Applications

Fangling Ji, Jun Ren, CÃ©cile Vincke, Lingyun Jia, and Serge Muyldermans

2. Overview, Generation, and Significance of Variable New Antigen Receptors (VNARs) as a Platform for Drug and Diagnostic Development

Samata S. Pandey, Marina Kovaleva, Caroline J. Barelle, and Obinna C. Ubah

Part II: Isolation of Single-Domain Antibodies

3. Llama DNA Immunization and Isolation of Functional Single-Domain Antibody Binders

FrÃ©dÃ©ric Trempe, Martin A. Rossotti, Tahir Maqbool, C. Roger MacKenzie, and Mehdi Arbabi-Ghahroudi

4. Preparation of Immune and Synthetic VNAR Libraries as Sources of High-Affinity Binders

Jahaziel Gasperin-Bulbarela, Olivia Cabanillas-Bernal, Salvador DueÃ±as, and Alexei F. Licea-Navarro

5. Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targets

Kaitlyn Bacon, Stefano Menegatti, and Balaji M. Rao

6. A Transgenic Heavy Chain IgG Mouse Platform as a Source of High Affinity Fully Human Single-Domain Antibodies for Therapeutic Applications

Dubravka Drabek, Rick Janssens, Rien van Haperen, and Frank Grosveld

Part III: Expression of Single-Domain Antibodies

7. Cytoplasmic Production of Nanobodies and Nanobody-Based Reagents by Co-Expression of Sulfhydryl Oxidase and DsbC Isomerase

Ario de Marco

8. Small Scale Secretory VHH Expression in Saccharomyces cerevisiae

Michiel M. Harmsen, Marga van Hagen-van Setten, and Peter T.J. Willemsen

9. Production of Single-Domain Antibodies in Pichia pastoris

Yusei Matsuzaki, Kaho Kajiwara, Wataru Aoki, and Mitsuyoshi Ueda

10. Production of Designer VHH-Based Antibodies in Plants

Henri De Greve

Part IV: Single-Domain Antibody Engineering and Characterization

11. Assessing the Aggregation Propensity of Single-Domain Antibodies Upon Heat-Denaturation Employing the ΔT_m Shift

Patrick Kunz

12. Facile Affinity Maturation of Single-Domain Antibodies Using Next-Generation DNA Sequencing

Michael J. Lowden, Henk van Faassen, Shalini Raphael, Shannon Ryan, Greg Hussack, and Kevin A. Henry

13. Engineering pH-Sensitive Single-Domain Antibodies

Tosha M. Laughlin and James R. Horn

14. Humanization of Camelid Single-Domain Antibodies

Traian Sulea

15. Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues

Paul J. Wichgers Schreur, Sandra van de Water, and Jeroen Kortekaas

16. Introducing Cysteines into Nanobodies for Site-Specific Labelling

Simon Boje Hansen and Kasper RÃ¸jkjÃ¦r Andersen

17. Affinity-Guided Site Selective Labelling of Nanobodies With Aldehyde Handles

Anders MÃ¤rcher and Kurt V. Gothelf

18. Cytoplasmic Expression of Nanobodies with Formylglycine Generating Enzyme Tag and Conversion to a Bio-Orthogonal Aldehyde Group

Da Li, Qiang Peng, Chungdong Huang, Berlin Zang, Jun Ren, Fangling Ji, Serge Muyldermans, and Lingyun Jia

19. Site-Specific Fluorescent Labelling, Single-Step Immunocytochemistry, and Delivery of Nanobodies into Living Cells

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