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1. RNA Modifications: A Mechanism that Modulates Gene Expression John Karijolich, Athena Kantartzis, and Yi-Tao Yu 2. Quantitative Analysis of RNA Modifications John Karijolich, Athena Kantartzis, and Yi-Tao Yu 3. Advances in RNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA Interference Mouldy Sioud 4. Progress in siRNA Delivery: A Multifunctional Approach by Polymeric Nanoparticles Weiwei Gao, Zeyu Xiao, Alex Radovic-Moreno, Jinjun Shi, Robert Langer, and Omid C. Farokhzad 5. Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells Anne Mobergslien and Mouldy Sioud 6. Systemic Delivery of Synthetic siRNAs Dag R. Sørensen and Mouldy Sioud 7. What Are the Key Targeted Delivery Technologies of siRNA Now? Mouldy Sioud 8. Using OligoWalk to Identify Efficient siRNA Sequences David H. Mathews 9. Jet-Injection of Short Hairpin RNA Encoding Vectors into Tumor Cells Wolfgang Walther, Ulrike Stein, and Hermann Lage 10. Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of Gene Knockdown Chris B. Moore, Elizabeth H. Guthrie, Max Tze-Han Huang, and Debra Taxman 11. Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1 Sheena M. Saayman, Patrick Arbuthnot, and Marc S. Weinberg 12. Functional Studies on RNA-Transfected Cell Microarrays Christina Sæten Fjeldbo, Kristine Misund, Clara-Cecilie Günther, Mette Langaas, Tonje Strømmen Steigedal, Liv Thommesen, Astrid Lægreid, and Torunn Bruland 13. Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migration Reiko Onuki-Nagasaki, Akira Nagasaki, Kazumi Hakamada, Taro Q.P. Uyeda, Satoshi Fujita, Masato Miyake, and JunMiyake 14. Intron-Mediated RNA Interference, Intronic MicroRNAs, and Applications Shao-Yao Ying, Chen Pu Chang, and Shi-Lung Lin 15. Inhibition of the microRNA Pathway in Zebrafish by siRNA Anders Fjose and Xiao-Feng Zhao 16. Profiling of miRNA Expression and Prediction of Target Genes Mouldy Sioud 17. Small RNA Cloning Seungil Ro and Wei Yan 18. In situ Hybridization Detection of microRNAs Rui Song, Seungil Ro, and Wei Yan 19. Detection and Quantitative Analysis of Small RNAs by PCR Seungil Ro and Wei Yan 20. In Vivo Reprogramming of Human Telomerase Reverse Transcriptase (hTERT) by Trans-Splicing Ribozyme to Target Tumor Cells Sang-Jin Lee, Seong-Wook Lee, Jin-Sook Jeong, and In-Hoo Kim 21. Design and Function of Triplex Hairpin Ribozymes Guillermo Aquino-Jarquin, Ramiro Rojas-Hernández, and Luis Marat Alvarez-Salas 22. Catalytic M1GS RNA as an Antiviral Agent in Animals Yong Bai, Paul Jay Fannin Rider, and Fenyong Liu 23. Using Live Cells to Generate Aptamers for Cancer Study Ling Meng, Kwame Sefah, Dalia Lopez Colon, Hui Chen, Meghan O'Donoghue, Xiangling Xiong, and Weihong Tan 24. Primer-Free Aptamer Selection Using a Random DNA Library Weihua Pan and Gary A. Clawson 25. Development of TLR 7/8 Small RNA Antagonists Mouldy Sioud 26. Modulation of Dendritic Cell Maturation and Function by siRNA-Bearing 5'-Triphosphate Mouldy Sioud 27. Immunotherapy of Cancer with Dendritic Cells Loaded with Tumor Antigens and Activated through mRNA Electroporation An M.T. Van Nuffel, Jurgen Corthals, Bart Neyns, Carlo Heirman, Kris Thielemans, and Aude Bonehill 28. Non-MHC-Dependent Redirected T Cells Against Tumor Cells Hilde Almåsbak, Marianne
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1. RNA Modifications: A Mechanism that Modulates Gene Expression John Karijolich, Athena Kantartzis, and Yi-Tao Yu 2. Quantitative Analysis of RNA Modifications John Karijolich, Athena Kantartzis, and Yi-Tao Yu 3. Advances in RNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA Interference Mouldy Sioud 4. Progress in siRNA Delivery: A Multifunctional Approach by Polymeric Nanoparticles Weiwei Gao, Zeyu Xiao, Alex Radovic-Moreno, Jinjun Shi, Robert Langer, and Omid C. Farokhzad 5. Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells Anne Mobergslien and Mouldy Sioud 6. Systemic Delivery of Synthetic siRNAs Dag R. Sørensen and Mouldy Sioud 7. What Are the Key Targeted Delivery Technologies of siRNA Now? Mouldy Sioud 8. Using OligoWalk to Identify Efficient siRNA Sequences David H. Mathews 9. Jet-Injection of Short Hairpin RNA Encoding Vectors into Tumor Cells Wolfgang Walther, Ulrike Stein, and Hermann Lage 10. Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of Gene Knockdown Chris B. Moore, Elizabeth H. Guthrie, Max Tze-Han Huang, and Debra Taxman 11. Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1 Sheena M. Saayman, Patrick Arbuthnot, and Marc S. Weinberg 12. Functional Studies on RNA-Transfected Cell Microarrays Christina Sæten Fjeldbo, Kristine Misund, Clara-Cecilie Günther, Mette Langaas, Tonje Strømmen Steigedal, Liv Thommesen, Astrid Lægreid, and Torunn Bruland 13. Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migration Reiko Onuki-Nagasaki, Akira Nagasaki, Kazumi Hakamada, Taro Q.P. Uyeda, Satoshi Fujita, Masato Miyake, and JunMiyake 14. Intron-Mediated RNA Interference, Intronic MicroRNAs, and Applications Shao-Yao Ying, Chen Pu Chang, and Shi-Lung Lin 15. Inhibition of the microRNA Pathway in Zebrafish by siRNA Anders Fjose and Xiao-Feng Zhao 16. Profiling of miRNA Expression and Prediction of Target Genes Mouldy Sioud 17. Small RNA Cloning Seungil Ro and Wei Yan 18. In situ Hybridization Detection of microRNAs Rui Song, Seungil Ro, and Wei Yan 19. Detection and Quantitative Analysis of Small RNAs by PCR Seungil Ro and Wei Yan 20. In Vivo Reprogramming of Human Telomerase Reverse Transcriptase (hTERT) by Trans-Splicing Ribozyme to Target Tumor Cells Sang-Jin Lee, Seong-Wook Lee, Jin-Sook Jeong, and In-Hoo Kim 21. Design and Function of Triplex Hairpin Ribozymes Guillermo Aquino-Jarquin, Ramiro Rojas-Hernández, and Luis Marat Alvarez-Salas 22. Catalytic M1GS RNA as an Antiviral Agent in Animals Yong Bai, Paul Jay Fannin Rider, and Fenyong Liu 23. Using Live Cells to Generate Aptamers for Cancer Study Ling Meng, Kwame Sefah, Dalia Lopez Colon, Hui Chen, Meghan O'Donoghue, Xiangling Xiong, and Weihong Tan 24. Primer-Free Aptamer Selection Using a Random DNA Library Weihua Pan and Gary A. Clawson 25. Development of TLR 7/8 Small RNA Antagonists Mouldy Sioud 26. Modulation of Dendritic Cell Maturation and Function by siRNA-Bearing 5'-Triphosphate Mouldy Sioud 27. Immunotherapy of Cancer with Dendritic Cells Loaded with Tumor Antigens and Activated through mRNA Electroporation An M.T. Van Nuffel, Jurgen Corthals, Bart Neyns, Carlo Heirman, Kris Thielemans, and Aude Bonehill 28. Non-MHC-Dependent Redirected T Cells Against Tumor Cells Hilde Almåsbak, Marianne
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