RNA Tagging (e-book) (used book) | bookbook.eu

Description

Part I: In Situ Detection of RNA Molecules Using FISH Techniques

1. Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization

Thomas Dresselhaus and Andrea Bleckmann

2. Quantitative Fluorescence In Situ Hybridization Detection of Plant mRNAs with Single-Molecule Resolution

Kun Huang, Mona Batish, Chong Teng, Alex Harkess, Blake C. Meyers, and Jeffrey L. Caplan

3. Visualization of Endoplasmic Reticulum-Associated mRNA in Mammalian Cells

Jingze J. Wu and Alexander F. Palazzo

4. Simultaneous Detection of mRNA and Protein in S. cerevisiae by Single Molecule FISH and Immunofluorescence

Evelina Tutucci and Robert H. Singer

Part II: In Vivo Imaging of RNA Transport and Localization

5. Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More

Michael Ryckelynck

6. Visualization of Transiently Expressed mRNA in Plants Using MS2

Eduardo José Peña and Manfred Heinlein

7. New Generations of MS2 Variants and MCP Fusions to Detect Single mRNAs in Living Eukaryotic Cells

Xavier Pichon, Marie-Cécile Robert, Edouard Bertrand, Robert H. Singer, and Evelina Tutucci

8. In Vivo Imaging of Mobile mRNAs in Plants Using MS2

Kai-Ren Luo, Nien-Chen Huang, and Tien-Shin Yu

9. RNA Imaging with RNase-Inactivated Csy4 in Plants and Filamentous Fungi

David Burnett, Alexander Lichius, and Jens Tilsner

Part III: Imaging and Analysis of RNA Uptake and Transport between Cells

10. Utilizing Potato Virus X to Monitor RNA Movement

Zhiming Yu, Sung Ki Cho, Pengcheng Zhang, Yiguo Hong, and David J. Hannapel

11. A Protocol for Non-Biased Identification of RNAs Transferred between Heterologous Mammalian Cell Types Using RNA Tagging, Cell Sorting, and Sequencing

Sandipan Dasgupta and Jeffrey E. Gerst

12. Synthesizing Fluorescently Labeled dsRNAs and sRNAs to Visualize Fungal RNA Uptake

Rachael Hamby, Ming Wang, Lulu Qiao, and Hailing Jin

13. Labeling of dsRNA for Fungal Uptake Detection Analysis

Matteo Galli, Jafargholi Imani, and Karl-Heinz Kogel

Part IV: Identification and Analysis of RNA-Binding Proteins

14. Using RNA Affinity Purification Followed by Mass Spectrometry to Identify RNA-Binding Proteins (RBPs)

Mengge Shan and Brian D. Gregory

15. Plant Individual Nucleotide Resolution Crosslinking and Immunoprecipitation to Characterize RNA-Protein Complexes