Ras Activity and Signaling (e-book) (used book) | bookbook.eu

Description

Part I: Generic Ras Biology

1. Ras Variant Biology and Contributions to Human Disease

Ian A. Prior

2. Regulation of the Small GTPase Ras and its Relevance to Human Disease

Part II: Methods in Ras Biochemistry

3. Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry

Lauren M. Adams, Caroline J. DeHart, and Neil L. Kelleher

4. Absolute Quantitation of GTPase Protein Abundance

Fiona E. Hood, Yasmina M. Sahraoui, Rosalind E. Jenkins, and Ian A. Prior

5. Validation of Isoform- and Mutation-specific RAS Antibodies

Andrew M. Waters and Channing J. Der

6. Production and Membrane Binding of N-terminally Acetylated, C-terminally Farnesylated and Carboxymethylated KRAS4b

Simon Messing, Constance Agamasu, Matt Drew, Caroline DeHart, Andrew G. Stephen, and William K. Gillette

7. Active GTPase Pulldown Protocol

Martin J. Baker and Ignacio Rubio

8. Methods to Monitor Ras Activation State

Kari Kopra and Harri Härmä

9. NMR Detection Methods for Profiling RAS Nucleotide Cycling

Ryan C. Killoran and Matthew J. Smith

Part III: Methods in Ras Processing, Trafficking, and Localization

Orit Gutman, Marcelo Ehrlich, and Yoav I. Henis

11. Understanding Ras Spatial Cycles through Reaction-Diffusion Simulations

Malte Schmick and Philippe I. H. Bastiaens

12. Super-Resolution Imaging and Spatial Analysis of RAS on Intact Plasma Membrane Sheets

Yong Zhou and John F. Hancock

13. FLIM-FRET Analysis of Ras Nanoclustering and Membrane-Anchorage

Hanna Parkkola, Farid Ahmad Siddiqui, Christina Oetken-Lindholm, and Daniel Abankwa

14. Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometry

Natividad R. Fuentes, Michael L. Salinas, Xiaoli Wang, Yang-Yi Fan, and Robert S. Chapkin

15. Spatiotemporal Imaging of Small GTPase Activity using Conformational Sensors for GTPase Activity (COSGA)

Yao-Wen Wu

Part IV: Methods in Ras Signaling and Inhibition

16. Using BioID to Characterize the RAS Interactome

Hema Adhikari and Christopher M. Counter

17. Probing RAS function with Monobodies

Imran Khan and John P. O'Bryan

18. Detection of Endogenous RASSF1A Interacting Proteins

Howard Donninger, Desmond Harrell-Stewart, and Geoffrey J. Clark

19. Mathematical Modeling to Study KRAS Mutant-Specific Responses to Pathway Inhibition

Edward C. Stites

20. A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background

Konstantin Budagyan and Jonathan Chernoff

Carmen G. Lechuga, Marina Salmón, Guillem Paniagua, Carmen Guerra, Mariano Barbacid, Matthias Drosten

Part V: Methods in vivo Ras Biology

22. Generation of Patient-derived Colorectal Cancer Organoids for RAS Studies

Dirk Schumacher, Joseph L. Regan, Dorothea Przybilla, and Reinhold Schäfer

23. Ras-GEF Mouse Models for the Analysis of Ras Biology and Signaling