Pseudogenes (e-book) (used book) | bookbook.eu

Description

Part I: Introductory Chapters

1. Pseudogene Redux with New Biological Significance

Leonardo Salmena

2. Contribution of Pseudogenes to Sequence Diversity

Mauno Vihinen

Part II: Methods to Identify Pseudogenes

3. Computational Methods for Pseudogene Annotation Based on Sequence Homology

Paul M. Harrison

4. Computational Methods of Identification of Pseudogenes Based on Functionality: Entropy and GC Content

Evgeniy S. Balakirev, Vladimir R. Chechetkin, Vasily V. Lobzin, and Francisco J. Ayala

5. Computational Methods of Identification of Pseudogenes Based on Functionality: Hybridization of 18S rRNA and mRNA During Translation

Chuanhua Xing

6. Whole Genome Identification of Neutrally Evolving Pseudogenes Using the Evolutionary Measure dN/dS

Lisa O. Andrieux and David Torrents Arenales

7. Methods to Study the Occurrence and the Evolution of Pseudogenes Through a Phylogenetic Approach

Jacques Dainat and Pierre Pontarotti

Part III: Methods to Detect Transcribed Pseudogenes of a Parental Gene of Interest

8. Methods for Detecting Transcribed Pseudogenes: PCR on Regions of High Sequence Similarity Followed by Cloning and Sequencing

Wenyong Ding and Jianwu Dai

Part IV: High-Throughput Methods to Detect Transcribed Pseudogenes

9. RNA Amplification for Pseudogene Detection Using RNA-Seq

Stephen C.M. Tsoi and Michael K. Dyck

10. GENCODE Pseudogenes

Adam Frankish and Jennifer Harrow

11. Methods to Detect Transcribed Pseudogenes: RNA-Seq Discovery Allows Learning Through Features

Camilo Valdes and Enrico Capobianco

Part V: High-Throughput Methods to Detect Translated Pseudogenes

12. Proteomics Techniques for the Detection of Translated Pseudogenes

Nadia Ucciferri and Silvia Rocchiccioli

Part VI: Methods to Study Transcribed Pseudogenes

13. Pseudogenes as Competitive Endogenous RNAs: Target Prediction and Validation

Florian A. Karreth, Ugo Ala, Paolo Provero, and Pier Paolo Pandolfi

14. Pseudogenes: A Novel Source of Trans Acting Antisense RNAs

Per Johnsson, Kevin V. Morris, and Dan Grandér

15. Pseudogene-Derived Endogenous siRNAs and Their Function

Wen-Ling Chan and Jan-Gowth Chang

Part VII: Methods to Study Translated Pseudogenes

16. Methods to Study Translated Pseudogenes: In Vitro Translation, Fusion with a Tag/Reporter Gene, and Complementation Assay

Anne Parle-McDermott

Part VIII: Methods to Study Translated Pseudogenes

17. Targeted and Robust Amplification of Mitochondrial DNA in the Presence of Nuclear Encoded Mitochondrial Pseudogenes Using Φ29 DNA Polymerases

Jonci N. Wolff

18. Discrimination of Pseudogene and Parental Gene DNA Methylation Using Allelic Bisulphite Sequencing

Luke B. Hesson and Robyn L. Ward

19. Mutational Analysis of CYP21A2 Gene and CYP21A1P Pseudogene: Long Range PCR on Genomic DNA

Hsien-Hsiung Lee

20. PMS2 Gene Mutational Analysis: RNA-Based Direct cDNA Sequencing to Circumvent Pseudogene Interference

Katharina Wimmer and Annekatrin Wernstedt

21. Dealing with Pseudogenes in Molecular Diagnostics in the Next Generation Sequencing Era

Kathleen Claes and Kim De Leeneer