Protocols in in Vitro Hepatocyte Research (e-book) (used book) | bookbook.eu

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Part I: In Vitro Models of the Hepatocyte Life Cycle1. Isolation And Culture Of Mouse Hepatocytes: Gender-Specific Gene Expression Responses To Chemical Treatments

Lyndsey Houseman, Mina Edwards, Ian R. Phillips, and Elizabeth A. Shephard

2. Cryopreservation of Hepatocytes

Nicola J. Hewitt and Albert P. Li

3. Culture Conditions Promoting Hepatocyte Proliferation And Cell Cycle Synchronization

Anne Corlu and Pascal Loyer

4. Immortalized Human Hepatic Cell Lines For In Vitro Testing And Research Purposes

Eva Ramboer, Tamara Vanhaecke, Vera Rogiers, and Mathieu Vinken

5. Culture and Functional Characterization Of Human Hepatoma Hepg2 Cells

María Teresa Donato, Laia Tolosa, and María José Gómez-Lechón

6. Establishment And Characterization Of An In Vitro Model Of Fas-Mediated Hepatocyte Cell Death

Mathieu Vinken, Michaël Maes, Sara Crespo Yanguas, Joost Willebrords, Tamara Vanhaecke, and Vera Rogiers

7. Serum-Free Directed Differentiation Of Human Embryonic Stem Cells To Hepatocytes

Kate Cameron, Baltasar Lucendo-Villarin, Dagmara Szkolnicka, and David C. Hay

8. Human Skin-Derived Precursor Cells: Isolation, Expansion And Hepatic Differentiation

Joery De Kock, Robim M. Rodrigues, Karolien Buyl, Tamara Vanhaecke, Vera Rogiers

9. Generation Of Hepatocytes From Pluripotent Stem Cells For Drug Screening and Developmental Modeling

Richard L. Gieseck III, Ludovic Vallier, and Nicholas R.F. Hannan

10. Differentiation-Promoting Medium Additives For Hepatocyte Cultivation and

Cryopreservation

Varvara Gouliarmou, Olavi Pelkonen, and Sandra Coecke

11. Co-Culture and Long-Term Maintenance Of Hepatocytes

Merav Cohen, Gahl Levy, and Yaakov Nahmias

12. Primary Hepatocytes In Sandwich Culture

Janneke Keemink, Marlies Oorts, and Pieter Annaert

13. Establishing Liver Bioreactors For In Vitro Research

Sofia P. Rebelo, Rita Costa, Marcos F.Q. Sousa, Catarina Brito, and Paula M. Alves

14. Epigenetic Modifications As Anti-Dedifferentiation Strategy For Primary Hepatocytes In Culture

Jennifer Bolleyn, Joanna Fraczek, Vera Rogiers, and Tamara Vanhaecke

15. Transfection Of Primary Hepatocytes With Liver-Enriched Transcription Factors Using Adenoviral Vectors

Marta Benet, Ramiro Jover, and Roque Bort

Part II: In Vitro Methods to Probe Hepatocyte Functionality and Toxicity

16. Transcriptomics Of Hepatocytes Treated With Toxicants For Investigating Molecular Mechanisms Underlying Hepatotoxicity

Vaibhav Shinde, Regina Stöber, Harshal Nemade, Isaia Sotiriadou, Jürgen Hescheler, JanHengstler, and Agapios Sachinidis

17. Global Microrna Analysis In Primary Hepatocyte Cultures

Julian Krauskopf, Almudena Espín Pérez, Jos C. Kleinjans, and Theo M. de Kok

18. Mass Spectrometry-Based Proteomics For Relative Protein Quantification And Biomarker Identification In Primary Human Hepatocytes

Lisa Dietz and Albert Sickmann

19. Targeted Metabolomics For Homocysteine-Related Metabolites In Primary Hepatocytes

Irena Selicharová and Marek Kořínek

20. Measurement Of Cytochrome P450 Enzyme Induction And Inhibition In Human Hepatoma Cells

Robim M. Rodrigues, Joery De Kock, Tatyana Doktorova, Vera Rogiers, and Tamara Vanhaecke

21. Analysis Of Sinusoidal Drug Uptake Transporter Activities In Primary Human Hepatocytes

Marc Le Vée, Elodie Jouan, Claire Denizot, Yannick Parmentier, and Olivier Fardel

22. Measurement Of Albumin Secretion As Functionality Test In Primary Hepatocyte Cultures

Karolien Buyl, Joery De Kock, Jen