Protein Chromatography (e-book) (used book) | bookbook.eu

Description

Part I Overview Chapters

1. A Synopsis of Protein and their Purification
Dermot Walls, Gary Cooney, and Sinéad T. Loughran

2. Gel-Filtration Chromatography
Ciarán Ó'Fágáin, Philip M. Cummins, and Brendan F. O'Connor

3. Immunoaffinity Chromatography: Concepts and Applications
Jenny Fitzgerald, Paul Leonard, Elaine Darcy, Shikha Sharma, and Richard O'Kennedy

4. Avoiding Proteolysis During Protein PurificationBarry J. Ryan and Gary T. Henehan

5. Scale-Up of Protein Purification: Downstream Processing Issues
John Joseph Milne

6. Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources
William J.J. Finlay, Laird Bloom, Joanne Grant, Edward Franklin, Deirdre Ní Shúilleabháin, and Orla Cunningham

7. Protein Stability: Enhancement and Measurement
Ciarán Ó'Fágáin

8. Tagging Recombinant Proteins to Enhance Solubility and Aid Purification
Sinéad T. Loughran and Dermot Walls

Part II Protocols Chapters

9. Storage and Lyophilisation of Pure Proteins
Ciarán Ó'Fágáin and Keith Colliton

10. Differential Precipitation and Solubilization of Proteins
Barry J. Ryan and Gemma K. Kinsella

11. Ion-Exchange Chromatography: Basic Principles and Application
Philip M. Cummins, Keith D. Rochfort, and Brendan F. O'Connor

12. Protein Quantitation and Analysis of Purity
Eva M. Campion, Sinéad T. Loughran, and Dermot Walls

13. Purification of Proteins Fused to Maltose-Binding Protein
Mario Lebendiker and Tsafi Danieli

14. Purification of Poly-Histidine-Tagged Proteins
Sinéad T. Loughran, Ronan T. Bree, and Dermot Walls

15. Purification of Antibodies Using Affinity Chromatography
Elaine Darcy, Paul Leonard, Jenny Fitzgerald, Martin Danaher, Hui Ma, and Richard O' Kennedy

16. Optimized Generation of High-Affinity, High