Plant Proteostasis (e-book) (used book) | bookbook.eu

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Approaches to Determine Protein Ubiquitination Residue Types.- Immunoprecipitation of Cullin Ring Ligases (CRLs) in Arabidopsis Thaliana Seedlings.- Radioligand Binding Assays for Determining Dissociation Constants of Phytohormone Receptors.- Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes.- Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants.- Generation of Artificial N-end Rule Substrate Proteins In Vivo and In Vitro.- Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases.- SUMO Chain Formation by Plants Enzymes.- Kinetic Analysis of Plant SUMO Conjugation Machinery.- Expression, Purification, and Enzymatic Analysis Of Plant SUMO Proteases.- Quantitative Analysis of Subcellular Distribution of the SUMO Conjugation System by Confocal Microscopy Imaging.- Biochemical Analysis of Autophagy in Algae and Plants by Monitoring the Electrophoretic Mobility of ATG8.- Detection of Autophagy in Plants by Fluorescence Microscopy.- Protocols for Studying Protein Stability in a Arabidopsis Protoplast Transient Expression System.- Detection and Quantification of Protein Aggregates in Plants.- Determination of Protein Carbonylation and Proteasome Activity in Seeds.- Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-based Protein Profiling in Plants.-Use of a Phosphatidylinositol phosphate Affinity Chromatography (PIP - chromatography) for the Isolation of Proteins Involved in Protein Quality Control and Proteostasis Mechanisms in Plants.- In vivo Radiolabeling of Arabidopsis Leaves and Separation of Thylakoid Membrane Complexes by Blue Native PAGE.- Normalized Quantitative Western Blotting Based on Standardized Fluorescent Labeling.- Sequence Search and Comparative Genomic Analysis of SUMO-Activating Enzymes using CoGe.- Studying Evolutionary Dynamics of Gene Families Encoding SUMO-Activating Enzymes with SeaView and ProtTest.- Bioinformatics Tools for Exploring the SUMO Gene Network.