Multiprotein Complexes (e-book) (used book) | bookbook.eu

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Preface...Table of Contents...Contributing Authors...
Part I
1. Production of Multi-Subunit Membrane Protein ComplexesBurak V. Kabasakal, Qiyang Jiang, and Christiane Schaffitzel
2. Production of Multiprotein Complexes using the Baculovirus Expression System: Homology Based and Restriction Free Cloning Strategies for Construct DesignPaola Rossollillo, Olga Kolesnikova, Karim Essabri, Gala Ramon Zamorano, and Arnaud Poterszman
3. Tagging Proteins with Fluorescent Reporters using the CRISPR/Cas9 System and Double Stranded DNA DonorsSylvain Geny, Simon Pichard, Alice Brion, Jean-Baptiste Renaud, Sophie Jacquemin, Jean-Paul Concordet, and Arnaud Poterszman
4. Validation of the Production of Antibodies in Different Formats in the HEK 293 Transient Gene Expression SystemJens König, Michael Hust, and Joop van den Heuvel
5. The Intervening Removable Affinity Tag (iRAT) System for the Production of Recombinant Antibody FragmentsNorimichi Nomura, Yayoi Nomura, Yumi Sato, and So Iwata
6. Isolation of Artificial Binding Proteins (Affimer Reagents) for Use in Molecular and Cellular BiologyAnna A. S. Tang, Christian Tiede, Michael J. McPherson, and Darren C. Tomlinson
Part II
7. Interactions Measurements of Protein-DNA Complexes by Isothermal Titration Calorimetry (ITC) and Microscale Thermophoresis (MST)Amandine Gontier, Paloma F. Varela, Clément Nemoz, Virginie Ropars, Magali Aumont-Nicaise, Michel Desmadril, and Jean-Baptiste Charbonnier
8. switchSENSE Technology for Analysis of DNA Polymerase KineticsGuillaume Bec and Eric Ennifar
9. Sedimentation Velocity Methods for Characterization of Protein Heterogeneity and Protein Affinity InteractionsChristine Ebel and Catherine Birck
10. Hands on Native Mass Spectrometry Analysis of Multi-Protein ComplexesStéphane Erb, Sarah Cianférani, and Julien Marcoux
11. Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass SpectrometryRuzena Filandrova, Daniel Kavan, Alan Kadek, Petr Novak, and Petr Man
12. Integrative Mass Spectrometry-Based Approaches for Modeling Macromolecular AssembliesAndy M. Lau and Argyris Politis
13. Optimization of Sample Preparation for the Observation of Macromolecular Complexes by Electron (cryo-)MicroscopyAlexandre Frechard, Grigory Sharov, Maximilien Werderer, and Patrick Schultz
14. Solubilization and Stabilization of Native Membrane Proteins for Drug DiscoveryCorvest Vincent and Jawhari Anass
Part III
15. Practical Aspects of Super-Resolution Imaging and Segmentation of Macromolecular Complexes by dSTORMLeonid Andronov, Jean-Luc Vonesch, and Bruno P. Klaholz
16. Multicolor FRET-FLIM Microscopy to Analyze Multiprotein Interactions in Live CellsAbdullah Ahmed, Jennifer Schoberer, Emily Cooke, and Stanley W. Botchway 17. Context-Specific and Proximity-Dependent Labeling for the Proteomic Analysis of Spatiotemporally Defined Protein Complexes with Split-BioIDCinthia Amaya Ramirez, Stefanie Egetemaier, and Julien Béthune
18. A Directed Evolution System for Lysine DeacetylasesMartin Spinck, Maria Ecke, Damian Schiller, and Heinz Neumann
Subject Index List...