Micrornas and the Immune System (e-book) (used book) | bookbook.eu

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MicroRNAs and the Immune System: Methods and Protocols Editor Silvia Monticelli Table of Contents: Table of Contents Preface Contributors Analysis of miRNA expression: 'classic' methods revisited 1. A rapid, quantitative assay for direct detection of microRNAs and other small RNAs using splinted ligation Sangpen Chamnongpol, Patricia A. Maroney and Timothy W. Nilsen 2. Normalization of microRNA Quantitative RT -PCR Data in Reduced Scale Experimental Designs Gary J. Latham 3. MicroRNA Detection in Bone Marrow Cells by LNA-FISH Silvana Debernardi and Amanda Dixon-McIver 4. Measuring microRNA expression in size-limited F ACS-sorted and microdissected samples Kai P Höfig and Vigo Heissmeyer High-throughput analysis of miRNAs 5. MicroRNA cloning from cells of the immune system Haoquan Wu, Joel Neilson and N. Manjunath 6. High-throughput profiling in the hematopoietic system Muller Fabbri, Riccardo Spizzo and George A. Calin 7. Construction of small RNA cDNA libraries for deep sequencing Molly F. Thomas and K. Mark Ansel 8. microRNA-profiling in formalin-fixed paraffin-embedded specimens Ulrich Lehmann Functional analysis of miRNAs in the immune system: gain-of-function 9. Expression of miRNAs in lymphocytes: a review Raquel Malumbres and Izidore S. Lossos 10. Mouse models for miRNA expression: the ROSA26 locus Stefano Casola 11. Regulation of monocytopoiesis by microRN As Laura Fontana, Antonio Sorrentino and Cesare Peschle 12. MicroRNA activity in B lymphocytes Virginia G. de Yébenes and Almudena R. Ramiro 13. The isolation and characterization of microRNAs of human mature erythrocytes Carolyn Sangokoya, Gregory LaMonte and Jen-Tsan Chi 14. Stable overexpression of miRNAs in bone marrow derived murine mast cells using lentiviral expression vectors Ramon J. Mayoral and Silvia Monticelli 15. Monitoring microRNA activity and validating microRNA targets by reporter-based approaches Alessia Baccarini and Brian D. Brown Functional analysis ofmiRNAs in the immune system: loss-of-function 16. Lentivirus-mediated antagomir expression Ewa Surdziel, Matthias Eder, and Michaela Scherr miRNA post-transcriptional modifications and mechanisms of action 17. Solution structure ofmiRNA: mRNA complex Mirko Cevec and Janez Plavec 18. MiRNA editing Dylan E. Dupuis and Stefan Maas Bioinformatic analysis and target prediction 19. Computational prediction of microRNA targets Xiaowei Wang 20. Large-scale integration of microRNA and gene expression data for identification of enriched microRNA: mRNA associations in biological systems. Preethi H. Gunaratne, Chad Creighton, Michael Watson and Jayantha B. Tennakoon miRNA and viruses 21. Identification and validation of the cellular targets of virus-encoded microRNAs Kin-Hang Kok, Ting Lei and Dong-Yan Jin