Melanoma (e-book) (used book) | bookbook.eu

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1. Model Systems for the Study of Malignant Melanoma

Randal K. Gregg

Part I: 2D Cell Culture-Based Approaches for Studying Melanoma Biology

2. Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editing

Kristian M. Hargadon, David Z. Bushhouse, Coleman E. Johnson, and Corey J. Willams

3. A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrix

Ewa Mazurkiewicz, Ewa MrÃ³wczyńska, Aleksandra Simiczyjew, Dorota Nowak, and Antonina J. Mazur

4. Wound Healing Assay for Melanoma Cell Migration

Juliano T. Freitas, Ivan Jozic, and Barbara Bedogni

5. A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells

Jelena Grahovac, Marijana Pavlovic, and Marija Vidosavljevic

6. Analyzing Melanoma Cell Oxygen Consumption and Extracellular Acidification Rates Using Seahorse Technology

Ashley V. Menk and Greg M. Delgoffe

7. Analysis of Melanoma Cell Glutamine Metabolism by Stable Isotope Tracing and Gas Chromatography-Mass Spectrometry

David A. Scott

8. Determination of Cytotoxic Activities Against Melanoma Cells Using Flow Cytometry

Guilan Shi and Richard Heller

9. In Vitro Differentiation of Tumor-Associated Macrophages from Monocyte Precursors with Modified Melanoma-Conditioned Medium

Tao Wang and Russel E. Kaufman

10. A Flow Cytometric Assay for Investigating Melanoma Cell Adhesion to Lymphatic Endothelial Cells

Kristian M. Hargadon and Coleman E. Johnson

Part II: 3D Cell Culture Systems for Studying Melanoma

11. An Approach to Study Melanoma Invasion and Crosstalk with Lymphatic Endothelial Cell Spheroids in 3D Using Immunofluorescence

Sanni Alve, Silvia Gramolelli, and PÃ¤ivi M. Ojala

12. Evaluating Melanoma Viability and Proliferation in 3D Microenvironments

Vasanth Siruvallur Murali, Murat Can Cobanoglu, and Erik S. Welf

13. Preparation, Drug Treatment, and Immunohistological Analysis of Tri-Culture Spheroid 3D Melanoma-Like Models

Maximilian E.A. SchÃ¤fer, Julia Klicks, Mathias Hafner, and RÃ¼diger Rudolf

14. Enrichment of Melanoma Cancer Stem Cells via Sphere Assays

Nabanita Mukherjee, Karoline A. Lambert, David A. Norris, and Yiqun G. Shellman

Part III: Techniques for Isolating and Studying Circulating Melanoma Cells

15. Capture and Isolation of Circulating Melanoma Cells Using Photoacoustic Flowmetry

Robert H. Edgar, Justin Cook, M

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