Malaria Immunology (e-book) (used book) | bookbook.eu

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Part I. Collecting, growing, monitoring, and manipulating Plasmodium falciparum parasites 1. Identification and enumeration of Plasmodium falciparum parasites by light microscopy Augustina Frimpong, Frederica D Partey and Michael F Ofori 2. Collection and cryopreservation of Plasmodium falciparum clinical isolates in the field Frederica D Partey, Augustina Frimpong and Michael F Ofori 3. Preservation of parasite RNA in the field Bernard Tornyigah and Nicaise Tuikue Ndam 4. Preservation and extraction of malaria parasite DNA from dried blood spots Helle Hansson, Queen Saidi and Michael Alifrangis 5. Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum Mary Lopez-Perez and Zakaria Seidu 6. Genotyping of Plasmodium falciparum isolates to assess clone composition in parasite cultures Ioanna Broumou, David F Plaza and Anna Färnert 7. Immunomagnetic selection of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants Mary Lopez-Perez and Rebecca W Olsen 8. Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants Mary Lopez-Perez 9. Selecting Plasmodium falciparum infected erythrocytes for adhesion to cell lines Yvonne Azasi and J Alexandra Rowe 10. Synchronisation of Plasmodium falciparum and P. knowlesi in vitro cultures using a highly specific protein kinase inhibitor Margarida Ressurreição, Robert W Moon, David A Baker, Christiaan van Ooij 11. Generation of Plasmodium falciparum gametocytes in vitro with specific considerations for field isolates Bismarck Dinko, Richmond Tackie, Gisela Henriques, Lindsay Stewart and Colin Sutherland 12. Imaging of extracellular vesicles derived from Plasmodium falciparum-infected red blood cells using atomic force microscopy Irit Rosenhek-Goldian, Paula Abou Karam, Neta Regev-Rudzki and Alicia Rojas Part II. Plasmodium falciparum gene transcription and manipulation of parasite genomes 13. Analysis of var gene transcript patterns by quantitative real-time PCR Anna Bachmann and Thomas Lavstsen 14. Analysis of var gene transcription pattern using DBLα-tags Kivisi C Andisi and Abdirahman I Abdi 15. RNAseq of infected erythrocyte surface antigen-encoding genes Hanh Hong Thi Nguyen, Suffian Azizan, Lee Ming Yeoh, Jingyi Tang and Michael F Duffy 16. Resetting var gene transcription in Plasmodium falciparum Vera Mitesser and Ron Dzikowski 17. CRISPR/Cas9 editing of the Plasmodium falciparum genome Sophie Adjalley and Marcus CS Lee 18. CRISPR-Cas9 editing of the Plasmodium falciparum genome - Special applications Xu Zhang, Kirk W Deitsch and Ron Dzikowski Part III. Plasmodium falciparum-infected erythrocyte surface antigens 19. Extraction and immunoprecipitation of VAR2CSA, the PfEMP1 associated with placental malaria Benoit Gamain and Dominique Dorin-Semblat 20. Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system Maria del Pilar Quintana 21. Expression of large full-length PfEMP1 proteins in HEK293 cells Jonathan P Renn, Justin YA Doritchamou and Patrick E Duffy 22. Receptor affinity-based purification of PfEMP1 proteins Rebecca W Olsen, Jennifer Suurbaar and Anja R Jensen 23. Analysis of antibody reactivity to malaria antigens by microsphere-based multiplex immunoassay Isobel S Walker, Amy WT Chung, T Damelang and Stephen J Rogerson 24. High-throughput BioPlex assay for the