Intrinsically Disordered Proteins (e-book) (used book) | bookbook.eu

Description

Part I: Sequence Properties

1. Disorder for Dummies: Functional Mutagenesis of Transient Helical Segments in Disordered Proteins

Gary W. Daughdrill

Christopher J. Oldfield, Xiao Fan, Chen Wang, A. Keith Dunker^, and Lukasz Kurgan

3. Computational Prediction of Disordered Protein Motifs using SLiMSuite

4. How to Annotate and Submit a Short Linear Motif to the Eukaryotic Linear Motif Resource

Marc Gouw, Jesús AlvaradoValverde, Jelena Čalyseva, Francesca -------Diella, Manjeet Kumar, Sushama Michael, Kim Van Roey, Holger Dinkel, and Toby J. Gibson

Garrett M. Ginell and Alex S. Holehouse

6. Exploring Protein Intrinsic Disorder with MobiDB

Alexander Miguel Monzon, András Hatos, Marco Necci, Damiano Piovesan, and Silvio C. E. Tosatto

Part II: Evolution

7. An Easy Protocol for Evolutionary Analysis of Intrinsically Disordered Proteins
Janelle Nunez-Castilla and Jessica Siltberg-Liberles

Part III: Production

8. Expression and Purification of an Intrinsically Disordered Protein

Karamjeet K. Singh and Steffen P. Graether

9. Production of Intrinsically Disordered Proteins for Biophysical Studies; Tips and Tricks

Christian Parsbæk Pedersen, Pernille Seiffert, Inna Brakti, and Katrine Bugge

10. Recombinant Production of Monomeric Isotope-Enriched Aggregation-Prone Peptides: Polyglutamine Tracts and Beyond

Albert Escobedo, Giulio Chiesa, and Xavier Salvatella

11. Cell-free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy

Part IV: Dynamics, Ensembles, and Structures

12. Structural Analyses of Intrinsically Disordered Proteins by Small-Angle X-ray Scattering

Amin Sagar, Dmitri Svergun, and Pau Bernadó

13. Determining Rg of IDPs from SAXS data
Ellen Rieloff and Marie Skepö

14. Obtaining Hydrodynamic Radii of Intrinsically Disordered Protein Ensembles by Pulsed Field Gradient NMR Measurements

Sarah Leeb and Jens Danielsson

Jakob T. Nielsen and Frans A. A. Mulder

16. Determination of pK_a Values in Intrinsically Disordered Proteins

Brandon Payliss and Anthony Mittermaier

17. Paris-DÉCOR: A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates

Rupashree Dass and Frans A.A. Mulder

Part V: Ensembles by Computation

18. Predicting Conformational Properties of Intrinsically Disordered Proteins from Sequence

Kiersten M. Ruff

19. Enhanced Molecular Dynamics Simulations of Intrinsically Disordered Proteins

Matteo Masetti, Mattia Bernetti, and Andrea Cavalli

20. Computational Protocol for Determining Conformational Ensembles of Intrinsically Disordered Proteins

Robert B. Best

21. Computing, Analyzing and Comparing the Radius of Gyration and Hydrodynamic Radius in Confo