Insoluble Proteins (e-book) (used book) | bookbook.eu

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1. General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins

Neus Ferrer-Miralles, Paolo Saccardo, José Luis Corchero, Zhikun Xu, and Elena García-Fruitós

Part I: Recombinant Protein Production in Escherichia coli

2. Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production

Agustín Correa and Pablo Oppezzo

3. Optimization of Culture Parameters and Novel Strategies to Improve Protein Solubility

Ranjana Arya, Jamal S.M. Sabir, Roop S. Bora, and Kulvinder S. Saini

4. Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purification

Zhanglin Lin, Qing Zhao, Bihong Zhou, Lei Xing, and Wanghui Xu

5. Beyond the Cytoplasm of Escherichia coli: Localizing Recombinant Proteins Where You Want Them

Jason T. Boock, Dujduan Waraho-Zhmayev, Dario Mizrachi, and Matthew P. DeLisa

6. Characterization of Amyloid-Like Properties in Bacterial Intracellular Aggregates

Anna Villar-Pique, Susanna Navarro, and Salvador Ventura

Part II: Strategies to Produce Insoluble Proteins in Cell-Free Expression Systems

7. Co-Translational Stabilization of Insoluble Proteins in Cell-Free Expression Systems

Lei Kai, Erika Orbán, Erik Henrich, Davide Proverbio, Volker Dötsch, and Frank Bernhard

Part III: Recombinant Protein Production in Lactic Acid Bacteria (LAB)

8. Functional Expression of Plant Membrane Proteins in Lactococcus lactis

Sylvain Boutigny, Emeline Sautron, Annie Frelet-Barrand, Lucas Moyet, Daniel Salvi, Norbert Rolland, and Daphné Seigneurin-Berny

Part IV: Recombinant Protein Production in Yeast

9. High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range of "Difficult-to-Express" Secretory Recombinant Proteins

Yasuaki Kawarasaki, Takeshi Kurose, and Keisuke Ito

Part V: Recombinant Protein Production in Insect Cells-Baculovirus

10. Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins

Judit Osz-Papai, Laura Radu, Wassim Abdulrahman, Isabelle Kolb-Cheynel, Nathalie Troffer-Charlier, Catherine Birck, and Arnaud Poterszman

Part VI: Recombinant Protein Production in Mammalian Cells

11. Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins

Joanne E. Nettleship, Peter J. Watson, Nahid Rahman-Huq, Louise Fairall, Mareike G. Posner, Abhishek Upadhyay, Yamini Reddivari, Jonathan M.G. Chamberlain, Simon E. Kolstoe, Stefan Bagby, John W.R. Schwabe, and Raymond J. Owens

12. Recombinant Glycoprotein Production in Human Cell Lines

Kamilla Swiech, Marcela Cristina Corrêa de Freitas, Dimas Tadeu Covas, and Virgínia Picanço-Castro

Part VII: Recombinant Protein Production in Other Systems

13. Soluble Recombinant Protein Production in Pseudoaltermonas haloplanktis TAC125

Maria Giuliani, Ermenegilda Parrilli, Filomena Sannino, Gennaro Apuzzo, Gennaro Marino, and Maria Luisa Tutino

Part VIII: Insoluble Protein Purification

14. A Screening Methodology for Purifying Proteins with Aggregation Problems

Mario Lebendiker, Michal Maes, and Assaf Friedler

15. Solubilization and Refolding of Inclusion Body Proteins

Anupam Singh, Vaibhav Upadhyay, and Amulya K. Panda

16. Bacterial Inclusion Body Purification

Joaquin Seras-Franzoso, Spela Peternel, Olivia Cano-Garrido, Antonio Villaverde, and Elena García-Fr