Insoluble Proteins (e-book) (used book) | bookbook.eu

Description

1. General Introduction: Recombinant protein production and purification of insoluble proteins

Neus Ferrer-Miralles, Paolo Saccardo, José Luis Corchero, and Elena García-Fruitós

Part I: Prokaryotic Expression Systems

2. Overcoming the solubility problem in E. coli: available approaches for recombinant protein production

Claudia Ortega, Pablo Oppezzo, and Agustín Correa

3. A3D 2.0 update for the prediction and optimization of protein solubility

Jordi Pujols, Valentín Iglesias, Jaime Santos, Aleksander Kuriata, Sebastian Kmiecik, and Salvador Ventura

4. Tailoring codon usage to the underlying biology for protein expression optimization

Zahra Alirezaeizanjani, Jan-Hendrik Trösemeier, Christel Kamp, and Sophia Rudorf

5. Protein Expression Optimization Strategies in E. coli: A Tailored Approach in Strain Selection and Parallelizing Expression Conditions

Shyn Ric Tang, Balaji Somasundaram, and Linda H.L. Lua

6. A novel approach for production of aggregation prone proteins using the spidroin-derived NT* tag

Nina Kronqvist, Anna Rising, and Jan Johansson

7. Cleavable Self-Aggregating Tags (cSAT) for Therapeutic Peptide Expression and Purification

Xiaofeng Yang, Zhanglin Lin, and Yanyun Jing

8. Use Intein Cleavable Polyhydroxyalkanoate Synthase Fusions to Improve Protein Solubility

Shuxiong Chen and Bernd Rehm

9. Introduction of a hexalysine (6K) tag can protect from N-terminal cleavage and increase yield of recombinant proteins expressed in the periplasm of E. coli

Vasiliki Paraskevopoulou, Mohammed Alissa, Naim Hage, and Franco H. Falcone

10. Two-tiered selection and screening strategy to increase functional enzyme production in Escherichia coli

Jason T. Boock, May Taw, Brian C. King, Robert J. Conrado, Donna M. Gibson, and Matthew P. DeLisa

Susheel K. Singh and Vandana Singh

12. Heterologous protein production in Lactobacillus (plantarum) using pSIP vectors

Geir Mathiesen, Lars Axelsson, Vincent GH Eijsink

13. Soluble recombinant protein production in Pseudoaltermonas haloplanktis TAC125: the case study of the full-length human CDKL5 protein

Marzia Calvanese, Andrea Colarusso, Concetta Lauro, Ermenegilda Parrilli and Maria Luisa Tutino

14. A protocol to enhance soluble protein expression in the cytoplasm of Bacillus subtilis

Hoang D Nguyen and Trang TP Phan

15. Applications of cell-free synthesized membrane protein precipitates

Julija Mezhyrova, Karsten Mörs, Clemens Glaubitz, Volker Dötsch, and Frank Bernhard

Part II: Eukaryotic Expression Systems

16. High cell-density expression system: yeast cells in a phalanx efficiently produce a certain range of "difficult-to-express" secretory recombinant proteins

Yasuaki Kawarasaki, Takeshi Kurose, Sayaka Ohashi, Runa Watabe, Mizuki Tanaka, and Keisuke Ito

17. Insect Cells-Baculovirus system for the production of difficult to express proteins: from expression screening for soluble constructs to protein quality control

Simon Pichard, Nathalie Troffer-Charlier, Isabelle Kolb-Cheynel, Pierre Poussin-Courmontagne, Wassim Abdulrahman, Catherine Birck, Vincent Cura, and Arnaud Poterszman

18. Transient gene expression in human Expi293 cells

Haroldo Cid da Silva Junior