DISSERTATION
Submitted to the Punjab Agricultural University in partial fulfillment of the requirements for the degree of
DOCTOR OF PHILOSOPHY IN MICROBIOLOGY.
ABSTRACT
Eight strains of yeasts, namely
S. cerevisiae-220, S. cerevisiae-360, S. cerevisiae-181/3c, Trichosporon beigelli, Candida haemulonii, C. parapsilosis, Candida sp.-3(1) and
Candida sp.-5(2) have been evaluated for their potential to ferment cane sugars to ethanol at three temperatures.
S. cerevisiae-220 utilized 84.0% substrate in 60 h at 30 ñ 1 ðC.
S. cerevisiae-360 fermented substrate more efficiently (69.3%) at 38 ñ 1 ðC.
S. cerevisiae-220 multiply faster at 30 ñ 1 as well as at 38 ñ 1 ðC.
S. cerevisiae-220 could withstand 13.0% ethanol whereas
S. cerevisiae-360 tolerated only 8.0% of ethanol supplemented in the growth medium. The protoplasts of
S. cerevisiae-220 and
S. cerevisiae-360 fused together could not generate ethanol more efficiently than their parents at 38 ñ 1 ðC.
S. cerevisiae-220 adapted at 38 ñ 1 ðC utilized 64.4% of substrate during 60 h.
Wine with commercially outstanding quality has been prepared from
Zingiber officianale with
S. cerevisiae-220-A at 38 ñ 1 ðC.
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