Human Retroviruses (e-book) (used book) | bookbook.eu

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Part I: Mapping the HIV Life Cycle

1. Virion Attachment & Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand Binding Assay

Claudia Cicala and James Arthos

2. CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5α

Gongpu Zhao and Peijun Zhang

3. The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating

Yang Yang, Jeremy Luban, and Felipe Diaz-Griffero

4. The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells

Thomas Hope

5. Imaging HIV-1 Nuclear Pre-Integration Complexes

Anna Cereseto and Mauro Giacca

6. HIV-1 Reverse Transcription

Andrea Cimarelli and Jean-Luc Darlix

7. RNase H: Specificity, Mechanisms of Action, and Antiviral Target

Karin Moelling, Felix Broecker, and John E. Kerrigan

8. HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat

Laurence Colin, Eric Verdin, and Carine Van Lint

9. HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export

Alan Cochrane

10. HIV-1 Accessory Proteins: Nef

Anke Heigele, Daniel Sauter, Jan Münch, and Frank Kirchhoff

11. HIV-1 Accessory Proteins: VpR

Richard Y. Zhao and Michael I. Bukrinsky

12. HIV-1 Accessory Proteins: Vpu and Vif

Amy Andrew and Klaus Strebel

13. SIV_SM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells

Gregory Berger and Andrea Cimarelli

14. Imaging of HIV Assembly and Release

Barbara Müller and Jacomine Krijnse-Locker

Part II: Isolation, Co-Receptor Use, and Cell Tropism of HIV-1

15. HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells

Stefania Dispinseri, Elisa Saba, Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, and Gabriella Scarlatti

16. Determination of HIV-1 Co-Receptor Usage

Mariangela Cavarelli and Gabriella Scarlatti

17. The Macrophage and HIV: Basic Concepts and Methodologies

Suzanne Gartner

18. HIV Infection of Dendritic Cells

Najla Nasr, Andrew Harman, Stuart Turville and Anthony L. Cunningham

19. Histocultures (Tissue Explants) in Human Retrovirology

Anush Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, Christophe Vanpouille, and Leonid Margolis

Part III: In Vivo Quantification of HIV-1

20. Single Copy Quantification of HIV-1 in Clinical Samples

Ann Wiegand and Frank Maldarelli

21. Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells

Christine Rouzioux, Adeline Mélard, and Véronique Avéttand-Fénoël

Part IV: Biotechnological Aspects of HIV-1

22. HIV-1 Based Lentiviral Vectors

Ying Poi Liu and Ben Berkhout

23. Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone

Zachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang

Part V: HTLVs

24. Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques

Greta Forlani, Roberto S. Accolla, and Giovanna Tosi

25. Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR

Claudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni

26. Quantitative Analysis of Human T-Lymphotropic