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Human Respiratory Syncytial Virus: An Introduction.- RSV Growth and Quantification by Microtitration and qRT-PCR Assays.- Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay.- Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays.- Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cells.- Development of Human Monoclonal Antibodies against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique.- Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection.- Host Factors Modulating RSV Infection; Use of Small Interfering RNAs to Probe Functional Importance.- In Vitro Modelling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs).- Reverse Genetics of Respiratory Syncytial Virus.- Use of Minigenome Systems to Study RSV Transcription.- Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics.- A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellular Factors as Drug Targets.- MicroRNA Profiling from RSV Infected Biofluids, Whole Blood, and Tissue Samples.- Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus.- In Vivo Assessment of Airway Function in the Mouse Model.- Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus.
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Human Respiratory Syncytial Virus: An Introduction.- RSV Growth and Quantification by Microtitration and qRT-PCR Assays.- Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay.- Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays.- Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cells.- Development of Human Monoclonal Antibodies against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique.- Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection.- Host Factors Modulating RSV Infection; Use of Small Interfering RNAs to Probe Functional Importance.- In Vitro Modelling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs).- Reverse Genetics of Respiratory Syncytial Virus.- Use of Minigenome Systems to Study RSV Transcription.- Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics.- A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellular Factors as Drug Targets.- MicroRNA Profiling from RSV Infected Biofluids, Whole Blood, and Tissue Samples.- Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus.- In Vivo Assessment of Airway Function in the Mouse Model.- Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus.
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