Heterologous Expression of Membrane Proteins (e-book) (used book) | bookbook.eu

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Heterologous Expression of Membrane Proteins: Methods and Protocols Isabelle Mus-Veteau, Editor Table of Contents Preface Contributors 1. Heterologous expression of membrane proteins for structural analysis Isabelle Mus-Veteau 2. Production of membrane proteins in E. coli and L. lactis Bert Poolman and Eric R. Geertsma 3. Mammalian membrane receptors expression as inclusion bodies in E. coli Bernard Mouillac and Jean-Louis Banères 4. Expression of membrane proteins at the E. coli membrane for structural studies Manuela Zoonens and Bruno Miroux 5. Membrane protein expression in Lactococcus lactis Annie Frelet-Barrand, Sylvain Boutigny, Edmund R. S. Kunji and Norbert Rolland 6. Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae Olivier Joubert, Rony Nehme, Michel Bidet and Isabelle Mus-Veteau 7. Mammalian membrane proteins expression in baculovirus infected insect cells Céline Trometer and Pierre Falson 8. Expression of membrane proteins in Drosophila melanogaster S2 cells: production and analysis of a EGFP-fused G Protein-Coupled Receptor as a model Karl Brillet, Carlos A. Pereira, and Renaud Wagner 9. Membrane protein expression in the eyes of transgenic flies. Valérie Panneels and Irmgard Sinning 10. Expression of Mammalian Membrane Proteins in Mammalian Cells using Semliki Forest Virus Vectors Kenneth Lundstrom 11. Membrane Protein Expression in Cell-Free Systems Birgit Schneider, Friederike Junge, Vladimir A. Shirokov, Florian Durst, Daniel Schwarz, Volker Dötsch, Frank Bernhard 12. Practical considerations of membrane proteininstability for purification and crystallisation Christopher G. Tate 13. Membrane protein solubilization Katia Duquesne and James N. Sturgis 14. Amphipols and fluorinated surfactants: two alternatives to detergents for studying membrane proteins in vitro Cécile Breyton, Bernard Pucci and Jean-Luc Popot 15. Heterologous expression and affinity purification of eukaryotic membrane proteins in view of functional and structural studies: the example of the sarcoplasmic reticulum Ca2+-ATPase Delphine Cardi, Cédric Montigny, Bertrand Arnou, Marie Jidenko, Estelle Marchal, Marc le Maire and Christine Jaxel