Ewing Sarcoma (e-book) (used book) | bookbook.eu

Description

Part I: Introduction

1. Germline Variation and Somatic Alterations in Ewing Sarcoma

Mitchell J. Machiela and Thomas G. P. Grünewald

2. Western Blot Analysis in Ewing Sarcoma

Aruna Marchetto and Laura Romero-Pérez

Part II: Molecular Biology Techniques Applied To Ewing Sarcoma

3. Tissue Preservation and FFPE Samples: Optimized Nucleic Acids Isolation in Ewing Sarcoma

Laura Romero-Pérez and Thomas G. P. Grünewald

4. Liquid Biopsies in Ewing Sarcoma

Manuela Krumbholz and Markus Metzler

5. (Immuno-)histological Analysis of Ewing Sarcoma

David Marcilla, Isidro Machado, Thomas G. P. Grünewald, Antonio Llombart-Bosch, and Enrique de Álava

6. Molecular Approaches to Diagnosis In Ewing Sarcoma: Fluorescence in situ hybridization (FISH)

Marcel Trautmann and Wolfgang Hartmann

7. Molecular Approaches to Diagnosis in Ewing Sarcoma: RT-PCR

Carlos Rodríguez-Martín and Javier Alonso

8. Molecular Approaches to Diagnosis in Ewing Sarcoma: Targeted RNA Sequencing

Carmen Salguero-Aranda and Juan Diaz-Martin

Part III: Diagnostics in Ewing Sarcoma

9. Ewing-specific (re)expression Models

Maximilian M. L. Knott and Florencia Cidre-Aranaz

10. Analysis of Regulatory Sequences by Dual-luciferase Reporter Assays in Ewing Sarcoma

Tilman L. B. Hölting and Maximilian M. L. Knott

Part IV: Cellular biology and cell culture techniques

11. Proliferation Assessment by Trypan Blue Exclusion in Ewing Sarcoma

Cornelius Funk and Julian Musa

12. Drug Screening by Resazurin Colorimetry in Ewing Sarcoma

13. Analysis of Migration and Invasion in Ewing Sarcoma

Part V: Animal models in Ewing sarcoma

Miwa Tanaka and Takuro Nakamura

15. Tumor Growth Analysis of Ewing Sarcoma Cell Lines Using Subcutaneous Xenografts in Mice

Florencia Cidre-Aranaz and Shunya Ohmura

16. Metastasis Assessment in Ewing Sarcoma Using Orthotopic Xenografts

Roser López-Alemany and Oscar M. Tirado

17. Orthotopic Implants in Mice

18. Ewing Sarcoma PDX Models

D. Surdez, L. Landuzzi, K. Scotlandi, and M.C. Manara

Susana Pascoal, Sarah Grissenberger, Eva Scheuringer, Rita Fior, Miguel Godinho Ferreira, and Martin Distel

20. Main Repositories and Databases used in Ewing Sarcoma Research

Maria Debiec-Rychter

21. Chromatin Immunoprecipitation Followed by Next-Generation Sequencing (ChIP-seq) Analysis in Ewing Sarcoma

Gwenneg Kerdivel and Valentina Boeva

22. Epigenetic Analysis in Ewing Sarcoma

Jeremy M. Simon and Nicholas C. Gomez

23. Systems Biology Analysis for Ewing Sarcoma