Epiblast Stem Cells (e-book) (used book) | bookbook.eu

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Part I: The Spectrum of Mouse Primed Pluripotent Stem Cells

1. Establishment of Mouse Epiblast Stem Cells

Pierre Osteil

2. L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell

Hannah J. Glover, Rachel A. Shparberg, and Michael B. Morris

3. Generation of Epiblast-Like Cells

Federica Cermola, Eduardo J. Patriarca, and Gabriella Minchiotti

Part II: Toolkits to Characterize and Study Primed Stem Cells

4. Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence

V. Pragathi Masamsetti and Patrick P.L. Tam

5. Small Interfering RNA (siRNA) Transfection in Epiblast Stem Cells

Georgia R. Kafer

6. Measuring Endocytosis and Endosomal Uptake at Single Cell Resolution

Carolyn Sangokoya

7. 3D Immunofluorescent Image Colocalization Quantification in Mouse Epiblast Stem Cells

Joshua G. Dierolf, Andrew J. Watson, and Dean H. Betts

8. Flow Cytometric Characterization of Pluripotent Cell Protein Markers in Naïve, Formative, and Primed Pluripotent Stem Cells

Joshua G. Dierolf, Kristin Chadwick, Courtney R. Brooks, Andrew J. Watson, and Dean H. Betts

9. Exploring Chromatin Accessibility in Mouse Epiblast Stem Cells with ATAC-Seq

Nazmus Salehin, Nicole Santucci, Pierre Osteil, and Patrick P.L. Tam

10. A Reproducible and Dynamic Workflow for Analysis and Annotation of scRNA-Seq Data

Nader Aryamanesh

11. Complete Transcriptome Analysis by 5'-End Single-Cell RNA-Seq with Random Priming

Tsukasa Kouno, Piero Carninci, and Jay W. Shin

12. Global Proteomic Profiling of Embryonic Stem Cells Using iTRAQ Isobaric Tags with LC-MS/MS Quantification

Aseel Sharaireh, Anna L. Tierney, and Richard D. Unwin

13. Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-Like Cells

Federico Pecori, Hisatoshi Hanamatsu, Jun-ichi Furukawa, and Shoko Nishihara

Part III: Primed Stem Cells to Engineer Gastrulation Model

14. Generation of Gastruloids from Epiblast-Like Cells

Federica Cermola, Eduardo J. Patriarca, and Gabriella Minchiotti

15. Definitive Endoderm from EpiSC Aggregates in Matrigel

Hisato Kondoh and Mai Fujii

16. In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-Like Cells (PGCLCs)

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