Enzyme Engineering (e-book) (used book) | bookbook.eu

Description

Part I: Enzyme Libraries Preparation and Screening

1. Preparation of Soil Metagenome Libraries and Screening for Gene Specific Amplicons

Luke J. Stevenson, David F. Ackerley, and Jeremy G. Owen

2. Ultrahigh-Throughput Screening of Metagenomic Libraries Using Droplet Microfluidics

Davide Agostino Cecchini, Mercedes Sánchez-Costa, Alejandro H. Orrego, Jesús Fernández-Lucas, and Aurelio Hidalgo

3. Synthetic DNA Libraries for Protein Engineering toward Process Improvement in Drug Synthesis

Michele Tavanti

Part II: Directed Evolution

4. In Silico Prediction Methods for Site-Saturation Mutagenesis

Ge Qu and Zhoutong Sun

5. Recombination of Compatible Substitutions by 2GenReP and InSiReP

Haiyang Cui, Mehdi D. Davari, and Ulrich Schwaneberg

Part III: Semi-Rational and De Novo Design

6. Using the Evolutionary History of Proteins to Engineer Insertion-Deletion Mutants from Robust, Ancestral Templates Using Graphical Representation of Ancestral Sequence Predictions (GRASP)

Connie M. Ross, Gabriel Foley, Mikael Boden, and Elizabeth M.J. Gillam

7. Resurrecting Enzymes by Ancestral Sequence Reconstruction

Maria Laura Mascotti

8. Expression and In Vivo Loading of De Novo Proteins with Tetrapyrrole Cofactors

Paul Curnow and J.L. Ross Anderson

Part IV: Rational Design

9. Rational-Design Engineering to Improve Enzyme Thermostability

Vinutsada Pongsupasa, Piyanuch Anuwan, Somchart Maenpuen, and Thanyaporn Wongnate

10. Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents

Haiyang Cui, Markus Vedder, Ulrich Schwaneberg, and Mehdi D. Davari

11. In Silico Engineering of Enzyme Access Tunnels

Alfonso Gautieri, Federica Rigoldi, Archimede Torretta, Alberto Redaelli, and Emilio Parisini

12. Quantum-Mechanical/Molecular-Mechanical (QM/MM) Simulations for Understanding Enzyme Dynamics

13. Computational Enzyme Design at Zymvol

Emanuele Monza, Victor Gil, and Maria Fatima Lucas

Part V: Biocatalytic Process Development

14. Advanced Enzyme Immobilization Technologies: An Eco-Friendly Support, a Polymer-Stabilizing Immobilization Strategy, and an Improved Cofactor Co-Immobilization Technique

Ana I. Benítez-Mateos and Francesca Paradisi

15. Chemical Reaction Engineering to Understand Applied Kinetics in Free Enzyme Homogeneous Reactors