DNA Repair Protocols (e-book) (used book) | bookbook.eu

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1. DNA repair: Front and Center and Not Going Away!

Vilhelm A. Bohr

Part I. Identification of Repair Genes and Generation of Repair Mutants

2. Identification of Genes Required for Damage Survival using a Cell Based RNAi Screen against the Drosophila Genome

Dashnamoorthy Ravi and Alexander James Roy Bishop

3. Isolating Genes Involved with Genotoxic Drug Response in the Nematode Caenorhabditis elegans using Genome-wide RNAi Screening

Lone Vedel Schøler, Tine Hørning Møller, Steffen Nørgaard, Lotte Vestergaard and Anders Olsen.

4. Establishment of the DNA Repair-defective Mutants in DT40 Cells

Masamichi Ishiai, Emi Uchida, and Minoru Takata

Part II. Assessing DNA Damage and Repair

5. Electrophoretic Mobility Shift Assays for

Protein-DNA Complexes Involved in DNA Repair

Chun Tsai, PhD, Vaughn Smider, Byung Joon Hwang, and Gilbert Chu

6. The Comet Assay A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair

Günter Speit and Andreas Rothfuss

7. Detection of DNA Damage by Comet Fluorescence in situ Hybridization (Comet-FISH)

W. Schlörmann and M. Glei

8. Quantitative DNA Damage and Repair Measurement with the Yeast Comet Assay

Rui Oliveira and Björn Johansson

9. Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells using Quantitative PCR

Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, Bennett Van Houten

Part III. Repair of Incorrect or Damaged DNA Bases

10. In vitro DNA Mismatch Repair in Human Cells

Liya Gu, Charles M. Ensor, and Guo-Min Li

11. Eukaryotic DNA Mismatch Repair in vitro

Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, Yanbin Zhang

12. Immuno-slot Blot Assay for Detection of UVR-mediated DNA Damage

Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke

13. Quantification of DNA Photoproductions in Mammalian Cell DNAUsing Radioimmunoassay

Thomas R. Berton and David L. Mitchell

14. Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-mediated PCR

Ahmad Besaratinia and Gerd P. Pfeifer

15. Construction of Plasmids Containing Site-specific DNA Interstrand Crosslinks for Biochemical and Cell Biological Studies

Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Schärer

16. Replication-coupled DNA Interstrand Crosslink Repair in Xenopus Egg Extracts

Puck Knipscheer, Markus Räschle, Orlando D. Schärer, and Johannes C. Walter

17. In Vitro Base Excision Repair using Mammalian Cell Extracts

Jason L. Parsons and Grigory L. Dianov^,

18. Using Arabidopsis Cell Extracts to Monitor Repair of DNA Base Damage in vitro

Dolores Córdoba-Cañero, Teresa Roldán-Arjona and Rafael R. Ariza

19. In vitro monitoring of Base Excision Repair in Saccharomyces cerevisiae

Simone Moertl and Fred Ahne

20. Mitochondrial Base Excision Repair Assays

Ricardo Gredilla and Tinna Stevnsner

Part IV. DNA Strand Breakage and Repair

21. O-Hydroxylamine-Coupled Alkaline Gel Electrophoresis Assay for the Detection and Measurement of DNA Single-Strand Breaks

April M. Luke and Jun Nakamura

22. DNA Double Stand Break Damage and Repair Assessed by Pulse-field Gel
Electrophoresis

Helen E. Bryant

23. Quantitative Amplification of Single Stranded DNA (QAOS)

Eva-Maria Holstein and David Lydall

24. Simple, Non-radioactive Measurement of Single-stranded DNA at Telomeric, Sub-telomeri