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Bioconjugation Protocols
Bioconjugation Protocols
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Part I: Protein Conjugates 1. Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities Edward A. Lemke 2. Enzymatically-Catalyzed Conjugation of a Biodegradable Polymer to Proteins and Small Molecules Using Microbial Transglutaminase Ahmed Besheer, Thomas C. Hertel, Jörg Kressler, Karsten Mäder, and Markus Pietzsch 3. Synthesis of Drug/Dye-Incorporated Polymer-Protein Hybrids Sukanta Dolai, Wei Shi, Bikash Mondal, and Krishnaswa…
  • Publisher:
  • Year: 2016
  • Pages: 605
  • ISBN-10: 1493960962
  • ISBN-13: 9781493960965
  • Format: 17.8 x 25.4 x 3.2 cm, softcover
  • Language: English
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Part I: Protein Conjugates

1. Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities

Edward A. Lemke

2. Enzymatically-Catalyzed Conjugation of a Biodegradable Polymer to Proteins and Small Molecules Using Microbial Transglutaminase

Ahmed Besheer, Thomas C. Hertel, Jörg Kressler, Karsten Mäder, and Markus Pietzsch

3. Synthesis of Drug/Dye-Incorporated Polymer-Protein Hybrids

Sukanta Dolai, Wei Shi, Bikash Mondal, and Krishnaswami Raja

4. Dye/DNA Conjugates as Multiple Labels for Antibodies in Sensitive Fluorescence Immunoassays

Qin Zhang, Shengchao Zhu, and Liang-Hong Guo

5. Chemoselective Modification of Viral Proteins Bearing Metabolically Introduced 'Clickable' Amino Acids and Sugars

Partha S. Banerjee and Isaac S. Carrico

6. Preparation of Peptide and Other Biomolecular Conjugates through Chemoselective Ligations

Mathieu Galibert, Olivier Renaudet, Didier Boturyn, and Pascal Dumy

7. New Fluorescent Substrates of Microbial Transglutaminase and Its Application to Peptide Tag-Directed Covalent Protein Labeling

Noriho Kamiya and Hiroki Abe

8. Covalent Conjugation of Poly(Ethylene Glycol) to Proteins and Peptides: Strategies And Methods

Anna Mero, Chiara Clementi, Francesco M. Veronese, and Gianfranco Pasut

9. Extending the Scope of Site-Specific Cysteine Bioconjugation by Appending a Prelabeled Cysteine Tag to Proteins Using Protein Trans-Splicing

Tulika Dhar, Thomas Kurpiers, and Henning D. Mootz

Part II: Nucleic Acid Conjugates

10. Polyethylenimine Bioconjugates for Imaging and DNA Delivery In Vivo

Andrea Masotti and Francesco Pampaloni

11. Synthesis of a Glycomimetic Oligonucleotide Conjugate By 1,3-Dipolar Cycloaddition

Gwladys Pourceau, Albert Meyer, Jean-Jacques Vasseur, and François Morvan

12. Site-Specific DNA Labeling by Staudinger Ligation

Samuel H. Weisbrod, Anna Baccaro, and Andreas Marx

13. Improved Cellular Uptake of Antisense Peptide Nucleic Acids (PNAs) by Conjugation to a Cell-Penetrating Peptide and a Lipid Domain

Takehiko Shiraishi and Peter E. Nielsen

14. Synthesis of Oligonucleotide-Peptide Conjugates for Biomedical and Technological Applications

Anna Aviñó, Santiago Grijalvo, Sónia Pérez-Rentero, Alejandra Garibotti, Montserrat Terrazas, and Ramon Eritja

15. Amphiphilic DNA Block Copolymers: Nucleic Acid-Polymer Hybrid Materials for Diagnostics and Biomedicine

Jan Zimmermann, Minseok Kwak, Andrew J. Musser, and Andreas Herrmann

Part III: Glycosyl and Lipid Conjugates

16. Chemically Selective Liposome Surface Glyco-Functionalization

Hailong Zhang, Yong Ma, and Xue-Long Sun

17. Bioconjugation Using Mutant Glycosyltransferases for the Site-Specific Labeling of Biomolecules with Sugars Carrying Chemical Handles

Boopathy Ramakrishnan, Elizabeth Boeggeman, Marta Pasek, and Pradman K. Qasba

18. Lipid-Core-Peptide System for Self-Adjuvanting Synthetic Vaccine Delivery

Mariusz Skwarczynski and Istvan Toth

19. Coupling Carbohydrates to Proteins for Glycoconjugate Vaccine Development Using a Pentenoyl Group as a Co

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  • Publisher:
  • Year: 2016
  • Pages: 605
  • ISBN-10: 1493960962
  • ISBN-13: 9781493960965
  • Format: 17.8 x 25.4 x 3.2 cm, softcover
  • Language: English English

Part I: Protein Conjugates

1. Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities

Edward A. Lemke

2. Enzymatically-Catalyzed Conjugation of a Biodegradable Polymer to Proteins and Small Molecules Using Microbial Transglutaminase

Ahmed Besheer, Thomas C. Hertel, Jörg Kressler, Karsten Mäder, and Markus Pietzsch

3. Synthesis of Drug/Dye-Incorporated Polymer-Protein Hybrids

Sukanta Dolai, Wei Shi, Bikash Mondal, and Krishnaswami Raja

4. Dye/DNA Conjugates as Multiple Labels for Antibodies in Sensitive Fluorescence Immunoassays

Qin Zhang, Shengchao Zhu, and Liang-Hong Guo

5. Chemoselective Modification of Viral Proteins Bearing Metabolically Introduced 'Clickable' Amino Acids and Sugars

Partha S. Banerjee and Isaac S. Carrico

6. Preparation of Peptide and Other Biomolecular Conjugates through Chemoselective Ligations

Mathieu Galibert, Olivier Renaudet, Didier Boturyn, and Pascal Dumy

7. New Fluorescent Substrates of Microbial Transglutaminase and Its Application to Peptide Tag-Directed Covalent Protein Labeling

Noriho Kamiya and Hiroki Abe

8. Covalent Conjugation of Poly(Ethylene Glycol) to Proteins and Peptides: Strategies And Methods

Anna Mero, Chiara Clementi, Francesco M. Veronese, and Gianfranco Pasut

9. Extending the Scope of Site-Specific Cysteine Bioconjugation by Appending a Prelabeled Cysteine Tag to Proteins Using Protein Trans-Splicing

Tulika Dhar, Thomas Kurpiers, and Henning D. Mootz

Part II: Nucleic Acid Conjugates

10. Polyethylenimine Bioconjugates for Imaging and DNA Delivery In Vivo

Andrea Masotti and Francesco Pampaloni

11. Synthesis of a Glycomimetic Oligonucleotide Conjugate By 1,3-Dipolar Cycloaddition

Gwladys Pourceau, Albert Meyer, Jean-Jacques Vasseur, and François Morvan

12. Site-Specific DNA Labeling by Staudinger Ligation

Samuel H. Weisbrod, Anna Baccaro, and Andreas Marx

13. Improved Cellular Uptake of Antisense Peptide Nucleic Acids (PNAs) by Conjugation to a Cell-Penetrating Peptide and a Lipid Domain

Takehiko Shiraishi and Peter E. Nielsen

14. Synthesis of Oligonucleotide-Peptide Conjugates for Biomedical and Technological Applications

Anna Aviñó, Santiago Grijalvo, Sónia Pérez-Rentero, Alejandra Garibotti, Montserrat Terrazas, and Ramon Eritja

15. Amphiphilic DNA Block Copolymers: Nucleic Acid-Polymer Hybrid Materials for Diagnostics and Biomedicine

Jan Zimmermann, Minseok Kwak, Andrew J. Musser, and Andreas Herrmann

Part III: Glycosyl and Lipid Conjugates

16. Chemically Selective Liposome Surface Glyco-Functionalization

Hailong Zhang, Yong Ma, and Xue-Long Sun

17. Bioconjugation Using Mutant Glycosyltransferases for the Site-Specific Labeling of Biomolecules with Sugars Carrying Chemical Handles

Boopathy Ramakrishnan, Elizabeth Boeggeman, Marta Pasek, and Pradman K. Qasba

18. Lipid-Core-Peptide System for Self-Adjuvanting Synthetic Vaccine Delivery

Mariusz Skwarczynski and Istvan Toth

19. Coupling Carbohydrates to Proteins for Glycoconjugate Vaccine Development Using a Pentenoyl Group as a Co

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