Animal Cell Biotechnology (e-book) (used book) | bookbook.eu

Description

Part I. Cell Line Development and Selection

1. High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidic Sorter

Wong Cheng Lee, Ali Asgar. S. Bhagat, and Chwee Teck Lim

2. Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology

João Vidigal, Fabiana Fernandes, Ana S Coroadinha, Ana P Teixeira, and Paula M. Alves

3. Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine

Lukas Fliedl and Christian Kaisermayer

4. Scalable Transient Protein Expression

Henry C. Chiou, Sanjay Vasu, Chao Yan Liu, Isabel Cisneros, Meredith B. Jones, and Jon Zmuda

5. DNA Profiling and Characterisation of Animal Cell Lines

Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins

Part II. Techniques for Process Development

6. Tools for High-Throughput Process and Medium Optimization

Martin Jordan and Matthieu Stettler

7. Designing Media for Animal Cell Culture - CHO Cells, The Industrial Standard

Karlheinz Landauer

8. Feed Optimization in Fed-Batch Culture

Ana Rita Costa, Maria Elisa Rodrigues, Mariana Henriques, Rosário Oliveira, and Joana Azeredo

9. Screening and Optimization of Chemically-Defined Media and Feeds with Integrated and Statistical Approaches

Zhihua Xiao, Michelle Sabourin, Graziella Piras, and Stephen Gorfien

10. Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteins

Maria Elisa Rodrigues, Ana Rita Costa, Mariana Henriques, Joana Azeredo, and Rosário Oliveira

11. Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System

Steve R.C. Warr

Part III. Cell Characterisation and Analysis

12. Monitoring Cell Growth, Viability and Apoptosis

Michael Butler, Maureen Spearman, and Katrin Braasch

13. Metabolic Flux Estimation in Mammalian Cell Cultures

Chetan T. Goudar, Richard K. Biener, James M. Piret, and Konstantin B. Konstantinov

14. Quenching Methods for the Analysis of Intracellular Metabolites

Judith Wahrheit and Elmar Heinzle

15. NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactors

Nelly Aranibar and Michael D. Reily

16. 1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cells

Tiago M. Duarte, Nuno Carinhas, Ana Carina Silva, Paula M. Alves, and Ana P. Teixeira

Part IV. Cultivation Techniques

17. Microfluidic Perfusion Culture

Koji Hattori, Shinji Sugiura, and Toshiyuki Kanamori

18. Disposable Bioreactors for Inoculum Production and Protein Expression Regine Eibl, Christian Löffelholz, and Dieter Eibl

19. Configuration of Bioreactors

Dirk E. Martens, E. J. van den End, and Mathieu Streefland

20. Process Analytical Technology (PAT) in Insect and Mammalian Cell Culture Processes - Dielectric Spectroscopy and Focused Beam Reflectance Measurement (FBRM)

Damir Druzinec, Katja Weiss, Christiane Elseberg, Denise Salzig, Matthias Kraume, Ralf Pörtner, and Peter Czermak

21. Cell Assessment by Atline Microscopy

Alexander Babitzky, Patrick Lindner, and Thomas Scheper

22. Seed Train Optimization for Cell Culture

Björn Frahm

Part V. Special Applications

23. Vaccine Production - Upstream Processing with Adherent or Suspension Cell